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An improved comprehensive two-dimensional (LC×LC) HPLC system for the analysis of triacylglycerols was developed. In the first-dimension, a Ag(I)-coated cation exchanger (250mm×2.1mm, 5μm) was employed with a gradient from 100% MeOH to 6% MeCN in MeOH at 20μL/min. Using a 10-way valve with two switching loops, 1min sections of the first-dimension were introduced in the second-dimension consisting...
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