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Strong cation-exchange (SCX) HPLC has been systematically evaluated for the chromatography of basic compounds using an eluent of 80% methanol containing ammonium formate (0.02 M) with the pH adjusted to 2.45 with trifluoroacetic acid. Contrary to common perception SCX-HPLC is capable of high efficiencies (>60 000 plates/m) and good peak symmetries (A s typically <1.2). Retention shows...
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