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Preparative HSCCC (high-speed counter-current chromatography) could be applied for the isolation of 9′-cis-neoxanthin from a crude carotenoid extract of fresh spinach leaves. The separation was performed on a Pharma-Tech Research Corp. CCC 1000 with a solvent system composed of hexane:ethanol:water at a volume ratio of 5:5:4.5 at a flow rate of 3mL/min and at 850rpm, using the lower phase as mobile...
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