Lime is commonly applied on agricultural lands for ameliorating soil acidity. However, lime dissolution and its concomitant contribution to carbon dioxide (CO 2 ) fluxes with the addition of organic residues at varying temperatures in acidic soils are not well known. We conducted laboratory incubation experiments for 96days (20+/−1°C and 40+/−1°C) to quantify the priming effects of lime (0.46 and 0.92% w/w) and mulch (0.5% w/w) additions on carbon (C) release in a Chromic Luvisol. The C released from lime (δ 13 C of −8.67‰), mulch (−13.02‰) and soil (−25.2‰) was quantified using their distinct δ 13 C values. Total lime derived C in soils without mulch constituted approximately 32% and 17% of the total C fluxes at 20°C and 40°C, respectively. During the 96-day incubation period, in the absence of mulch addition between 64% and 100% of the applied lime C was released as CO 2 at both incubation temperatures. Furthermore, lime derived, mulch derived and soil derived C increased by 59, 48 and 284% respectively, when the incubation temperature was increased from 20°C to 40°C. These results suggest that mineralization of native soil C was more sensitive to temperature than the C released by lime dissolution and mulch mineralization. Temperature sensitivity of soil derived C was lower in treatments with mulch and lime addition compared to soil without C amendments. We obtained a Q 10 value of 2.41±0.06 for the C released by the lime dissolution in the acidic soil. These findings are important for modelling the contribution of different C sources to atmospheric CO 2 concentrations in soils, such as the acidic limed and mulched soils.