There is growing evidence that natural products are important in the relationship between nutrients and healthin humans. Currently, one of the biggest problems in the food industries is the waste management that is generated in food production. In the present work, we have obtained a persimmon extract (PE) rich in bioactive compounds from the industrial sub-product of natural persimmon juice. We present here the characterization of bioactive components of PE and document their effects in hypertrophic and insulin resistant adipocytes.High-performance liquid chromatography coupled to mass spectrometry (HPLC-ESI-MS) was used for detected and identified carotenoids such as α-carotene, β-carotene and β-criptoxantin and polyphenols such as quercetin, procyanidin, hesperidin/neohesperidin, among others. To determine the properties of the PE, the antioxidant status was measured by the TEAC, FOLIN, FRAP and ORAC tests, showing a strong antioxidant activity.The intracellular ROS generation and triglyceride accumulation was measured by sensitive fluorescent probes in a hypertrophied adipocyte model. The results show that the PE exhibited an effective dose-dependent decrease of intracellular ROS generation as well as triglyceride accumulation in hypertrophic adipocytes.Therefore, PE extract can be used for preventive purposes against oxidative stress and obesity.