The purpose of this study was to examine the influence of maximal strength capacity on muscle activation, during cycling, at three selected cadences: a low cadence (50rpm), a high cadence (110rpm) and the freely chosen cadence (FCC). Two groups of trained cyclists were selected on the basis of the different maximal isokinetic voluntary contraction values (MVCi) of their lower extremity muscles as follow: F min (lower MVCi group) and F max (higher MVCi group). All subjects performed three 4-min cycling exercises at a power output corresponding to 80% of the ventilatory threshold under the three cadences. Neuromuscular activity of vastus lateralis (VL), rectus femoris (RF) and biceps femoris (BF) was studied quantitatively (integrated electromyography, IEMG) and qualitatively (timing of muscle bursts during crank cycle). Cadence effects were observed on the EMG activity of VL muscle and on the burst onset of the BF, VL and RF muscles. A greater normalized EMG activity of VL muscle was observed for the F min group than the F max group at all cadences (respectively F min vs. F max at 50rpm: 17±5% vs. 38±6%, FCC: 22±7% vs. 44±5% and 110rpm: 21±6% vs. 45±6%). At FCC and 110rpm, the burst onset of BF and RF muscles of the F max group started earlier in the crank cycle than the F min group These results indicate that in addition to the cadence, the maximal strength capacity influences the lower extremity muscular activity during cycling.