A rapid, sensitive, robust and specific method was developed for the determination and quantitation of felodipine, in human blood plasma by liquid chromatography coupled with tandem mass spectrometry using nimodipine as internal standard. Felodipine was extracted from 0.5mL human plasma by use of a liquid/liquid procedure using diethyl ether/hexane (80/20, v/v) as eluent. The method included a chromatographic run of 5min using a C 18 analytical column (100mm×4.6mm i.d.) and the calibration curve was linear over the range from 0.02 to 10ngmL −1 (r 2 >0.994). The between-run precision, determined as relative standard deviation of replicate quality controls, was 5.7% (0.06ngmL −1 ), 7.1% (0.6ngmL −1 ) and 6.8% (7.5ngmL −1 ). The between-run accuracy was ± 0.0, 2.1 and 3.1% for the above-mentioned concentrations, respectively.