In-use micro-scale fuel use and emission rates were measured for eight cement mixer trucks using a portable emission measurement system. Each vehicle was tested on petroleum diesel and B20 biodiesel. Average fuel use and emission rates increase monotonically versus engine manifold absolute pressure. A typical duty cycle includes loading at a cement plant, transit while loaded from the cement plant to work site, creeping in a queue of vehicles at the worksite, unloading, and transit without load from the site to the plant. For B20 versus petroleum diesel, there is no significant change in the rate of fuel use, CO 2 emissions, and NO emissions, and significant decreases in emissions for CO, hydrocarbons, and particulate matter. For loaded versus unloaded onroad travel, fuel use and CO 2 emissions rates are approximately 60% higher and the rates for other pollutants are approximately 30–50% higher. A substantial portion of cycle emissions occurred at the work site. Inter-vehicle and intra-cycle variability are also quantified using the micro-scale methodology.