Indonesian potato growers face increasing problems from Phthorimaea operculella, whose larvae are responsible for damage in potato tubers. Use of biological control agents, and specifically entomopathogenic micro-organisms, could be an alternative method to chemical control. From this perspective, we carried out a screening and an evaluation of local granuloviruses which are naturally present in Indonesian pest populations. The use of an enzyme-linked immuno-sorbent assay allowed us to isolate three granulovirus strains from P. operculella larvae collected from three different locations in Indonesia: Wonsosobo (Central Java), Lembang (West Java), and Berastagi (Northern Sumatra). Bioassays did not reveal significant differences in the biological properties (LC 50 and ST 50 ) of these strains. The restriction pattern of the viral genome (Wonsosobo strain) presented only minor variations compared to other isolates collected in different regions of the world.