The ventilatory response to brief, severe hypoxia is biphasic consisting of an initial facilitation followed by a slowing of breathing frequency (fr). After the hypoxic stimulus is removed, fr drops below baseline levels. This phenomenon is called the post-hypoxic frequency decline (phfd). These fr changes are due to reciprocal changes in expiratory time (Te), mediated by the ventrolateral pontine A 5 region (J. Physiol. (London) 497 (1996) 79; Am. J. Physiol. 274 (1998) R1546). The purpose of this study was to determine if carotid body input is required for full manifestation of phfd by quantifying ventilation in intact and carotid sinus denervated rats in response to hypoxic, and contrasted with hypercapnic stimuli. Following carotid denervation the initial facilitation of fr was eliminated in response to hypoxia, but the phfd remained. In contrast the pattern in response to increased CO 2 remained constant before and after carotid denervation. These results suggest that phfd is not dependent upon carotid body stimulation, but is mediated centrally.