A simple and inexpensive ion-exchange chromatography method for the separation of medically useful no-carrier-added (nca) iodine radionuclides from bulk amounts of irradiated tellurium dioxide (TeO 2 ) target was developed and tested using 131 I. The radiochemical separation was performed using a very small Dowex-1×8 ion-exchange column. The overall radiochemical yield for the complete separation of 131 I was 92±1.8 (standard deviation) % (n=8). The separated nca 131 I was of high, ∼99%, radionuclidic and radiochemical purity and did not contain detectable amounts of the target material. This method may be adopted for the radiochemical separation of other different iodine radionuclides produced from tellurium matrices through cyclotron as well as reactor irradiation.