Since the designs of optimal formulations for resveratrol permeation via the skin are lacking, the aim of this study was to establish the profile of resveratrol permeability into and across human skin. For that, a laboratory-made chromatographic column was used (Zr-PMODS), with its performance being compared to a traditional C18 column. In vitro drug release was conducted with polysulfone membranes, and the flux (J S ) was 30.49μgcm −2 h −1 ), with a lag time (L T ) of 0.04h, following a pseudo-first-order kinetics. For ex vivo percutaneous absorption using excised female human skin, the kinetic profile was the same, but J S was 0.87μgcm −2 h −1 and L T was 0.97h. From the initials 49.30μg applied to the skin, 9.50μg were quantified in the receptor medium, 20.48μg was retained at the stratum corneum (do not account as permeated) and 21.41μg was retained at the viable epidermis+dermis (account as permeated), totalizing 30.90μg of resveratrol permeated after 24h of application (62.6%). From these results, one can conclude that a person using the 1-g emulsion dose released by the pump containing 20mg of resveratrol will have, theoretically, 12.53mg of it liberated into his bloodstream, gradually and continuously for 24h.