The cubic component of the distortion product otoacoustic emission (DPOAE) in response to two tones of frequency f 1 and f 2 is generated by so-called primary- and secondary-source mechanisms in the cochlea. Interference between the resulting two source components can limit the usefulness of DPOAEs in assessing cochlear function. Although techniques are available for separating the source components, depending on the application, they can be either time-consuming or ineffective without a priori knowledge of optimal parameters. Here, we investigated, in humans, the possibility of separating the source components in the time-domain by sampling the onset and offset of the DPOAE-time signal at appropriate instants. Therefore, a DPOAE paradigm was developed in which the f 2 tone was periodically switched on during the continuous presence of the f 1 tone. F 2 was increased in 20-Hz steps from 1.5 to 2.5kHz and the ratio f 2 /f 1 held constant at 1.2; measurements were made at six primary tone levels, ranging from L 2 =25 to 65dB SPL. To investigate the possibility of separating the two sources by appropriate sampling, we developed an algorithm called onset-decomposition. The algorithm is based on the shape properties of DP-grams constructed from DPOAE responses at different time instants in the onset of the DPOAE signal. Thus, at each such time instant, the source components were extracted by time-windowing of the corresponding DP-gram. The time courses of the amplitude onsets of these separated primary- and secondary-source components provided evidence that the primary-source component attained its steady-state before the secondary-source component started to significantly influence the DPOAE by interference with the primary-source component. Consequently, in the final paradigm, the primary-source component is extracted by sampling the DPOAE signal at a single pre-defined time instant after the onset of the f 2 stimulus tone, before the secondary component begins to interfere. Based on the near-absence of interference maxima and minima in the DP-grams, the appropriate sampling instant was 8–10ms for all frequencies and intensities in the stimulus set. Extracting the primary-source by onset sampling has the advantage that when individual source components for a given f 2 are to be investigated, there is no need to measure a DP-gram. In conclusion, it is shown that the technique can reliably and quickly separate the source components, making it an attractive paradigm for applications in basic research and clinical diagnosis.