This paper shows a Holocene paleoecological reconstruction based on a peat bog sequence (El Paují, 4°28′N–61°35′W, 865m elevation) located in the transition zone between the Gran Sabana (SE Venezuela) savannas and the Amazon rainforests. Paleoecological trends are based on the analysis of pollen and pteridophyte spores, algal and animal remains, fungal spores, and charcoal particles. The whole record embraces the last ca. 8000calyr BP, and was subdivided into five pollen zones, representing the following vegetation succession: savanna/rainforest mosaic (8250–7715yr BP), dense rainforests (7715–5040yr BP), savanna/rainforest mosaic (5040–2690yr BP), secondary dry forests (2690–1440yr BP), and peat bog in an open savanna landscape (1440yr BP–present). These vegetation changes have been attributed to the action of climate and/or land use changes, as well as the corresponding synergies between them. Fire has been determinant in the landscape evolution. Based on the reconstructed fire and vegetation shifts, a changing land use pattern could have been recognized. Between the early and the mid Holocene (ca. 8.3–5.0kyr BP), land use practices seem to have been more linked to shifting agriculture in a rainforest landscape – as is usual in Amazon cultures – with medium fire incidence affecting only local forest spots or surrounding savannas. More extensive forest burning was recorded between ca. 5.0 and 2.7kyr BP, followed by land abandonment and the dominance of drier climates between 2.7 and 1.4yr BP. The modern indigenous culture, which prefers open environments and makes extensive use of fire thus preventing forest re-expansion, seem to have established during the last 1500yr. Therefore, a significant cultural replacement has been proposed for the region, leading to the present-day situation. Changing human activities have been instrumental for ecological evolution in this savanna–rainforest transitional region, as well as for the shaping of modern landscapes.