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Two different RTi-PCR protocol were designed and quantifications were validated by using various amounts of DNA. Multiplex AGR1 and AGR2 RTi-PCR amplification reactions quantified successfully for Clostridium perfringens, Enterococcus faecalis, Staphylococcus aureus, Escherichia coli O157:H7 and Salmonella enterica. Using the validated multiplex RTi-PCR reactions, the presence and quantification of...
This study compared the current pig slaughter procedure where the pluck set is completely removed with a procedure where the pluck set is partially removed, leaving the highly contaminated oral cavity, tonsils and tongue untouched. The effect on carcass contamination was investigated by enumerating hygiene indicator bacteria (total aerobic count, Enterobacteriaceae and E. coli) and cefotaxime-resistant...
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