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In this study, we confirmed our previous hypothesis that the 24 kD thermostable skeletal muscle protein (TSMP) recognized by a panel of porcine-specific monoclonal antibodies (MAbs) is skeletal troponin I (sTnI). The TSMP and sTnI purified from porcine muscle have identical electrophoretic mobilities, isoelectric characteristics, and specific antigenicities. The heterogeneity of sTnI between porcine...
Regulatory controls to prevent the spread of BSE have prohibited the use of certain animal proteins in feed in several countries. Accurate analytical methods for detecting prohibited material in feedstuffs are needed to ensure compliance with the new regulations. Six IgG class monoclonal antibodies (MAbs) against troponin I (TnI), a thermostable marker protein, have been developed for the detection...
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