Stable isotope analyses of faunal remains provide valuable information about human–environment interactions in the past, including insights into past animal husbandry and land management strategies. Here, we report stable carbon (δ 13 C) and nitrogen (δ 15 N) isotope values of collagen and carbonate from archaeological fauna from Kałdus, a medieval settlement in North-Central Poland, to better understand human–environment interactions during a period of increasing urbanism and marketization. Wild and domestic animals can be separated on the basis of their isotopic values. The mean δ 15 N value for 12 domesticated animals is 7.6 ± 1.2‰ and for 5 wild animals is 4.3 ± 0.5‰ (p = 0.002). The mean collagen δ 13 C value for domesticated animals is −20.6 ± 1.1‰ and for wild animals is −22.0 ± 0.5‰ (p = 0.004). The mean carbonate δ 13 C value for domesticated animals is −13.14 ± 1.3‰ and for wild animals is −14.14 ± 0.9‰ (p = 0.034). The “canopy effect” and anthropogenic effects that alter stable isotope ratios of plants (manuring, swidden agriculture and ploughing) are discussed in relation to these differences. Fish are isotopically variable, which suggests broad-spectrum fishing strategies and/or trade, and increases our awareness of the difficulties in interpreting human paleodiet when freshwater fish were on the menu.