In recent years, an interest has grown with Y-chromosome single nucleotide polymorphisms (Y-SNPs) and their potential applications, especially in evolutionary biology, forensics and medical genetics. A total of 81 samples from unrelated males of the Brazilian population of São Paulo State (Ribeirão Preto) have been analyzed for 14 Y-SNPs (SRY1532, M213, M9, M70, M22, TAT, 92R7, M173, P25—multiplex 1; M170, M62, M172, M26 and M201—multiplex 2) using the SNaPshot™ methodology, and haplogroups frequencies were determined. European influence (mainly due to the male Portuguese settlers, but also the later arrival of Spanish and Italian) is therefore confirmed, given the fact that the most predominant European haplogroup R1b1 is also the most representative haplogroup (53.09%), followed by haplogroup J2 (with not more than 12.35%). The remaining haplogroups have made a minor contribution, between 9.88% and 1.23%, yet very significant.