Developmental Biology

Items from 1 to 20 out of 41 results

article

Differential Adhesion Leads to Segregation and Exclusion of N-Cadherin-Deficient Cells in Chimeric Embryos

Igor Kostetskii, Robert Moore, Rolf Kemler, Glenn L. Radice

Developmental Biology > 2001 > 234 > 1 > 72-79

Cadherin-mediated cell–cell interactions are thought to be critical in controlling cell sorting during embryogenesis. Here, we report that chimeric embryos generated with N-cadherin-deficient (N-cadherin^−/−) embryonic stem cells develop further than embryos completely lacking N-cadherin only when the myocardium consists of N-cadherin-positive cells. Initially, the N-cadherin-negative and -positive...

article

The Wheels Mutation in the Mouse Causes Vascular, Hindbrain, and Inner Ear Defects

Alireza Alavizadeh, Amy E. Kiernan, Patrick Nolan, Cecilia Lo, more

Developmental Biology > 2001 > 234 > 1 > 244-260

In a screen for mouse mutations with dominant behavioral anomalies, we identified Wheels, a mutation associated with circling and hyperactivity in heterozygotes and embryonic lethality in homozygotes. Mutant Wheels embryos die at E10.5–E11.5 and exhibit a host of morphological anomalies which include growth retardation and anomalies in vascular and hindbrain development. The latter includes perturbation...

article

Functions of the Growth Arrest Specific 1 Gene in the Development of the Mouse Embryo

K.K.H. Lee, A.K.C. Leung, M.K. Tang, D.Q. Cai, more

Developmental Biology > 2001 > 234 > 1 > 188-203

The growth arrest specific 1 (gas1) gene is highly expressed in quiescent mammalian cells (Schneider et al., 1988, Cell 54, 787–793). Overexpression of gas1 in normal and some cancer cell lines could inhibit G₀/G₁ transition. Presently, we have examined the functions of this gene in the developing mouse embryo. The spatial–temporal expression patterns for gas1 were established in 8.5- to 14.5-day-old...

article

Simultaneous Measurement of Intracellular Nitric Oxide and Free Calcium Levels in Chordate Eggs Demonstrates That Nitric Oxide Has No Role at Fertilization

Louise A. Hyslop, Michael Carroll, Victoria L. Nixon, Alex McDougall, more

Developmental Biology > 2001 > 234 > 1 > 216-230

At fertilization in sea urchin, the free radical nitric oxide (NO) has recently been suggested to cause the intracellular Ca²⁺ rise responsible for egg activation. The authors suggested that NO could be a universal activator of eggs and the present study was set up to test this hypothesis. Intracellular NO and Ca²⁺ levels were monitored simultaneously in eggs of the mouse or the urochordate ascidian...

article

Neural Induction in the Absence of Mesoderm: β-Catenin-Dependent Expression of Secreted BMP Antagonists at the Blastula Stage in Xenopus

Oliver Wessely, Eric Agius, Michael Oelgeschläger, Edgar M. Pera, more

Developmental Biology > 2001 > 234 > 1 > 161-173

A growing body of work indicates that neural induction may be initiated prior to the establishment of the gastrula mesodermal organizer. Here, we examine neural induction in Xenopus embryos in which mesoderm induction has been blocked by Cerberus-short, a reagent that specifically inhibits Nodal-related (Xnr) signals. We find that extensive neural structures with cyclopic eyes and brain tissue are...

article

Notochord Patterning of the Endoderm

Ondine Cleaver, Paul A Krieg

Developmental Biology > 2001 > 234 > 1 > 1-12

Endodermally derived organs of the gastrointestinal and respiratory system form at distinct anterioposterior and dorsoventral locations along the vertebrate body axis. This stereotyped program of organ formation depends on the correct patterning of the endodermal epithelium so that organ differentiation and morphogenesis occur at appropriate positions along the gut tube. Whereas some initial patterning...

article

Cell Type-Specific Expression of Fasciclin II Isoforms Reveals Neuronal–Glial Interactions during Peripheral Nerve Growth

Jay W. Wright, Philip F. Copenhaver

Developmental Biology > 2001 > 234 > 1 > 24-41

During the formation of the insect peripheral nervous system (PNS), the cell adhesion receptor fasciclin II has been shown to play a prominent role in axonal fasciculation and synapse formation during motor neuron outgrowth. In the moth Manduca, fasciclin II (MFas II) is expressed both as a transmembrane isoform (TM-MFas II) and a glycosyl phosphatidylinositol-linked isoform (GPI-MFas II). By using...

article

Lateral Line Placodes Are Induced during Neurulation in the Axolotl

Gerhard Schlosser, R.Glenn Northcutt

Developmental Biology > 2001 > 234 > 1 > 55-71

In order to determine the time window for induction of lateral line placodes in the axolotl, we performed two series of heterotopic and isochronic transplantations from pigmented to albino embryos at different stages of embryogenesis and assessed the distribution of pigmented neuromasts in the hosts at later stages. First, ectoderm from the prospective placodal region was transplanted to the belly...

article

Axis Induction by Wnt Signaling: Target Promoter Responsiveness Regulates Competence

Rachel S. Darken, Paul A. Wilson

Developmental Biology > 2001 > 234 > 1 > 42-54

The modulation of inductive competence is a major theme in embryonic development, but, in most cases, the underlying mechanisms are not well understood. In principle, the capacity of extracellular signals to elicit particular responses could be regulated by changes in cell surface receptors, in intracellular signaling pathways, or in the responsiveness of individual target gene promoters. As an example...

article

Vertebrate Proteins Related to Drosophila Naked Cuticle Bind Dishevelled and Antagonize Wnt Signaling

Keith A Wharton, Gregor Zimmermann, Raphaël Rousset, Matthew P Scott

Developmental Biology > 2001 > 234 > 1 > 93-106

Wnt signals control cell fate decisions and orchestrate cell behavior in metazoan animals. In the fruit fly Drosophila, embryos defective in signaling mediated by the Wnt protein Wingless (Wg) exhibit severe segmentation defects. The Drosophila segment polarity gene naked cuticle (nkd) encodes an EF hand protein that regulates early Wg activity by acting as an inducible antagonist. Nkd antagonizes...

article

NeuroD2 Is Necessary for Development and Survival of Central Nervous System Neurons

James M. Olson, Atsushi Asakura, Lauren Snider, Richard Hawkes, more

Developmental Biology > 2001 > 234 > 1 > 174-187

NeuroD2 is sufficient to induce cell cycle arrest and neurogenic differentiation in nonneuronal cells. To determine whether this bHLH transcription factor was necessary for normal brain development, we used homologous recombination to replace the neuroD2 coding region with a β-galactosidase reporter gene. The neuroD2 gene expressed the reporter in a subset of neurons in the central nervous system,...

article

SNARE-Dependent Signaling at the Drosophila Wing Margin

Bryan A. Stewart, Mahmood Mohtashami, Lily Zhou, William S. Trimble, more

Developmental Biology > 2001 > 234 > 1 > 13-23

The wing of Drosophila melanogaster has long been used as a model system to characterize intermolecular interactions important in development. Implicit in our understanding of developmental processes is the proper trafficking and sorting of signaling molecules, although the precise mechanisms that regulate membrane trafficking in a developmental context are not well studied. We have therefore chosen...

article

Positive and Negative Regulation of Epicardial–Mesenchymal Transformation during Avian Heart Development

Christopher J. Morabito, Robert W. Dettman, Javier Kattan, J.Michael Collier, more

Developmental Biology > 2001 > 234 > 1 > 204-215

In the developing heart, the epicardium is essential for coronary vasculogenesis as it provides precursor cells that become coronary vascular smooth muscle and perivascular fibroblasts. These precursor cells are derived from the epicardium via epithelial–mesenchymal transformation (EMT). The factors that regulate epicardial EMT are unknown. Using a quantitative in vitro collagen gel assay, we show...

article

Hox11 Acts Cell Autonomously in Spleen Development and Its Absence Results in Altered Cell Fate of Mesenchymal Spleen Precursors

Benoit Kanzler, T.Neil Dear

Developmental Biology > 2001 > 234 > 1 > 231-243

The genetic steps governing development of the spleen are largely unknown. Absence of Hox11 in mice results in asplenia, but it is unclear how Hox11 exerts its effect on spleen development. To more precisely define Hox11's role in spleen morphogenesis, we have examined the fate of the developing spleen in Hox11^−/− mice. Perturbation of spleen development begins between dE13 and dE13.5. Cells of the...

article

Bep4 Protein Is Involved in Patterning along the Animal–Vegetal Axis in the Paracentrotus lividus Embryo

Daniele P. Romancino, Giovanna Montana, Serena Dalmazio, Marta Di Carlo

Developmental Biology > 2001 > 234 > 1 > 107-119

In sea urchin embryos, the initial animal–vegetal (AV) axis is specified during oogenesis but the mechanism is largely unknown. By using chemical reagents such as lithium, it is possible to shift the principal embryonic territories toward a vegetal fate. We have investigated the possibility of obtaining the same morphological effect as with lithium by utilizing Fabs against the maternal Bep4 protein...

article

The BMP Family Member Gdf7 Is Required for Seminal Vesicle Growth, Branching Morphogenesis, and Cytodifferentiation

Stephen Settle, Paul Marker, Kyle Gurley, Abhishek Sinha, more

Developmental Biology > 2001 > 234 > 1 > 138-150

Epithelial–mesenchymal interactions play an important role in the development of many different organs and tissues. The secretory glands of the male reproductive system, including the prostate and seminal vesicles, are derived from epithelial precursors. Signals from the underlying mesenchyme are required for normal growth, branching, and differentiation of the seminal vesicle epithelium. Here, we...

article

Inwardly Rectifying K+ Channels in Spermatogenic Cells: Functional Expression and Implication in Sperm Capacitation

Carlos Muñoz-Garay, José L. De la Vega-Beltrán, Ricardo Delgado, Pedro Labarca, more

Developmental Biology > 2001 > 234 > 1 > 261-274

To fertilize, mammalian sperm must complete a maturational process called capacitation. It is thought that the membrane potential of sperm hyperpolarizes during capacitation, possibly due to the opening of K⁺ channels, but electrophysiological evidence is lacking. In this report, using patch-clamp recordings obtained from isolated mouse spermatogenic cells we document the presence of a novel K⁺-selective...

article

Lineage Analysis in the Chicken Inner Ear Shows Differences in Clonal Dispersion for Epithelial, Neuronal, and Mesenchymal Cells

Hainan Lang, Donna M. Fekete

Developmental Biology > 2001 > 234 > 1 > 120-137

The epithelial components of the vertebrate inner ear and its associated ganglion arise from the otic placode. The cell types formed include neurons, hair-cell mechanoreceptors, supporting cells, secretory cells that make endolymphatic fluid or otolithic membranes, and simple epithelial cells lining the fluid-filled cavities. The epithelial sheet is surrounded by an inner layer of connective and vascular...

article

Adenylyl Cyclase A Expression Is Tip-Specific in Dictyostelium Slugs and Directs StatA Nuclear Translocation and CudA Gene Expression

Irene Verkerke-van Wijk, Masashi Fukuzawa, Peter N Devreotes, Pauline Schaap

Developmental Biology > 2001 > 234 > 1 > 151-160

cAMP oscillations, generated by adenylyl cyclase A (ACA), coordinate cell aggregation in Dictyostelium and have also been implicated in organizer function during multicellular development. We used a gene fusion of the ACA promoter with a labile lacZ derivative to study the expression pattern of ACA. During aggregation, most cells expressed ACA, but thereafter expression was lost in all cells except...

article

Neurotrophins Are Not Required for Normal Embryonic Development of Olfactory Neurons

Serge Nef, Mark E. Lush, Tracey E. Shipman, Luis F. Parada

Developmental Biology > 2001 > 234 > 1 > 80-92

Neurons of the vertebrate olfactory epithelium (OE) regenerate continuously throughout life. The capacity of these neurons to regenerate and make new and precise synaptic connections in the olfactory bulb provides a useful model to study factors that may control or mediate neuronal regeneration. Expression and in vitro studies have suggested potential roles for the neurotrophins in the olfactory system...

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234
Year:
2001

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Keywords

MOUSE (3)
SEA URCHIN (3)
XENOPUS (3)
ADHESION (2)
CELL ADHESION (2)
COMPETENCE (2)
DROSOPHILA (2)
EGF-CFC (2)
EPH (2)
FERTILIZATION (2)
GASTRULATION (2)
GENE REGULATION (2)
HEART DEVELOPMENT (2)
HOMEOBOX (2)
MORPHOGENESIS (2)
NODAL (2)
SIGNAL TRANSDUCTION (2)
TRANSCRIPTION FACTOR (2)
WINGLESS (2)
ACTIVATION (1)
ADDITIONAL SEX COMBS (1)
ADENYLYL CYCLASE A (1)
ALKALINE PHOSPHATASE (1)
AMBYSTOMA (1)
ANEUPLOIDY (1)
ANGIOBLAST (1)
ANGIOGENESIS (1)
ASCIDIAN (1)
ASCIDIAN EMBRYOGENESIS (1)
ASPLENIA (1)
ASYMMETRIC GENE EXPRESSION (1)
AV AXIS (1)
AVE (1)
AXIS INDUCTION (1)
AXOLOTL (1)
BDNF (1)
BEP (1)
BLASTOCYST (1)
BLOOD VESSEL ASSEMBLY (1)
BMP (1)
BMP4 (1)
CADHERIN (1)
CALCIUM (1)
CAMP OSCILLATIONS (1)
CARDIAC CONDUCTION SYSTEM (1)
CD31 (1)
CELL CYCLE (1)
CELL DEATH (1)
CELL MOVEMENT (1)
CELL REARRANGEMENT (1)
CEREBELLUM (1)
CFC1 (1)
CFC2 (1)
CHICK DEVELOPMENT (1)
CHICK EMBRYO FOREBRAIN (1)
CHICKEN (1)
CHICKEN EMBRYO (1)
CHORDIN (1)
CORONARY VASCULOGENESIS (1)
CROSS-LINKING (1)
CYCLIN (1)
CYSTS (1)
CYTOPLASMIC DETERMINANTS (1)
CYTOPLASMIC TRANSFER (1)
DAZ-LIKE (1)
DBHR (1)
DEVELOPMENT (1)
DICTYOSTELIUM (1)
DICTYOSTELIUM DISCOIDEUM (1)
DISHEVELLED (1)
DOPAMINE-Β-HYDROXYLASE (DBH) (1)
DOSAGE COMPENSATION (1)
DOSAGE EFFECTS (1)
DROSOPHILA. (1)
DSH (1)
DVL (1)
EGG (1)
EMBRYO (1)
EMBRYOGENESIS (1)
ENDODERM (1)
ENDODERM DIFFERENTIATION (1)
ENU MUTAGENESIS (1)
EPHA RECEPTORS (1)
EPHRIN B (1)
EPHRINS A (1)
EPICARDIUM (1)
EPITHELIAL–MESENCHYMAL TRANSFORMATION (1)
EYE (1)
FASCICULATION (1)
FAUST. (1)
FGF (1)
FOLLISTATIN (1)
GASTRULATION MOVEMENTS (1)
GATA (1)
GERM CELLS (1)
GERM PLASM (1)
GLO (1)
GOLGI COMPLEX (1)
GRADIENTS (1)
GROWTH ARREST SPECIFIC 1 GENE (1)
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Developmental Biology

Differential Adhesion Leads to Segregation and Exclusion of N-Cadherin-Deficient Cells in Chimeric Embryos

The Wheels Mutation in the Mouse Causes Vascular, Hindbrain, and Inner Ear Defects

Functions of the Growth Arrest Specific 1 Gene in the Development of the Mouse Embryo

Simultaneous Measurement of Intracellular Nitric Oxide and Free Calcium Levels in Chordate Eggs Demonstrates That Nitric Oxide Has No Role at Fertilization

Neural Induction in the Absence of Mesoderm: β-Catenin-Dependent Expression of Secreted BMP Antagonists at the Blastula Stage in Xenopus

Notochord Patterning of the Endoderm

Cell Type-Specific Expression of Fasciclin II Isoforms Reveals Neuronal–Glial Interactions during Peripheral Nerve Growth

Lateral Line Placodes Are Induced during Neurulation in the Axolotl

Axis Induction by Wnt Signaling: Target Promoter Responsiveness Regulates Competence

Vertebrate Proteins Related to Drosophila Naked Cuticle Bind Dishevelled and Antagonize Wnt Signaling

NeuroD2 Is Necessary for Development and Survival of Central Nervous System Neurons

SNARE-Dependent Signaling at the Drosophila Wing Margin

Positive and Negative Regulation of Epicardial–Mesenchymal Transformation during Avian Heart Development

Hox11 Acts Cell Autonomously in Spleen Development and Its Absence Results in Altered Cell Fate of Mesenchymal Spleen Precursors

Bep4 Protein Is Involved in Patterning along the Animal–Vegetal Axis in the Paracentrotus lividus Embryo

The BMP Family Member Gdf7 Is Required for Seminal Vesicle Growth, Branching Morphogenesis, and Cytodifferentiation

Inwardly Rectifying K+ Channels in Spermatogenic Cells: Functional Expression and Implication in Sperm Capacitation

Lineage Analysis in the Chicken Inner Ear Shows Differences in Clonal Dispersion for Epithelial, Neuronal, and Mesenchymal Cells

Adenylyl Cyclase A Expression Is Tip-Specific in Dictyostelium Slugs and Directs StatA Nuclear Translocation and CudA Gene Expression

Neurotrophins Are Not Required for Normal Embryonic Development of Olfactory Neurons

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