A complex spin-echo spectrum of 6 3 , 6 5 Cu and 2 0 9 Bi has been observed in various ceramic and single-crystal samples of Bi 2 CuO 4 in antiferromagnetic phase at a temperature of 4.2 K. The spectrum consists of 6 3 , 6 5 Cu AFNR and 2 0 9 Bi NQR with a complex structure of resonance lines due to magnetic inequivalency of Bi atoms and a large asymmetry parameter. The Cu AFNR is characterized by the internal field H i n t ~ 99 kOe at Cu nuclei and by the quadrupole frequency 6 3 v Q ~ 10 MHz. The obtained value of H i n t corresponds to 0.82 μ B for the Cu 2 + magnetic moment. It is shown that the low limit of the internal field at 2 0 9 Bi nuclei is approximately 4.3 kOe and consists of the dipolar field H d i p = 0.85 kOe and the hyperfine field H h f which essentially exceeds H d i p . The occurrence of H h f at 2 0 9 Bi nuclei shows that Bi 3 + anions take part in the superexchange interaction between Cu atoms along the Cu O Bi O Cu bonds.