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The mechanism by which glucose and other fuels stimulate phosphoinositide-specific phospholipase C (PLC) in pancreatic islet beta cells is not known. Previous studies have suggested that glucose may couple to PLC β1 and PLC δ1. To determine directly if fuels activate these PLC isozymes, clones stably overexpressing PLC β1 or PLC δ1 were generated in the fuel-sensitive beta cell line RINm5F, and secretagogue...
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