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A monomeric alanine aminopeptidase was purified to a single band in SDS–PAGE from bovine skeletal muscle by using a procedure including ammonium sulphate fractionation, adsorption on DEAE–cellulose, gel filtration on Ultrogel ACA 34, and adsorption on hydroxyapatite. Molecular weight determination by gel filtration and sodium dodecyl sulphate–polyacrylamide gel electrophoresis yielded a molecular...
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