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The lipid transfer and mixing properties in hydrated dispersions of dimyristoyl phosphatidylcholine (DMPC)/dimyristoyl glycerol (DMG) binary mixtures have been investigated by using electron spin resonance spectroscopy of the spin-labelled lipid components. The assay system is based on the reduction in spectral broadening from spin-spin interactions that takes place by dilution of the spin-labelled...
A phosphatidic acid phosphatase (PAP; EC 3.1.3.4.), dephosphorylating phosphatidic acid (PA) to diacylglycerol (DAG), was identified and purified from the plasma membrane of Acholeplasma laidlawii A. After four purification steps, including membrane preparation, Tween 20 solubilization, preparative gel electrophoresis and electro-elution, PAP was purified about 400 times to near homogeneity. The...
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