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Fluorescent styrylpyridinium dyes have recently been suggested as probes of the membrane dipole potential and of the kinetics of electrogenic ion pumps. It is necessary, however, to be able to confidently attribute observed fluorescence changes to electrical effects alone and avoid interference from changes in membrane fluidity. Furthermore, the effect of the dyes themselves on the dipole potential...
We investigated whether cell alkalinization via activation of Na + /H + exchange is involved in the stimulation of Na + conductance and Na + ,K + -ATPase in rat hepatocytes under hypertonic stress. Osmolarity was increased from 300 to 400 mOsm/l at constant extracellular pH (7.4), whereas osmotically induced cell alkalinization (0.3 pH units in HCO 3 ...
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