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The affinity of the human red cell nucleoside transporter for the transport inhibitor nitrobenzylthioinosine decreases upon protein purification. The affinity was highest for the whole cells (K d , 0.04 nM), lowered upon cytoskeleton depletion (K d , 0.2 nM) and lowest after partial purification and reconstitution (K d , 0.3 nM), as determined by frontal affinity chromatography.
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