Plant regeneration from callus of fan flower, Scaevola phlebopetala F. Muell., an Australian native species of horticultural significance has been achieved. The effects of coconut milk, kinetin, 2,4-D and BAP on callus and shoot induction were investigated. Coconut milk promoted callus induction. Kinetin was not required for callus induction and 2,4-D induced a poor quality callus. BAP had a significant effect on callus induction, growth and appearance of callus. BAP also induced shoot initiation from callus produced from root and leaf explants. Shoot regeneration was achieved on a medium containing 5.0 % coconut milk, 60 g/L sucrose and 3 mg/L BAP. Shoot growth required a hormone free medium containing 20 g/L sucrose. Regenerated shoots were rooted on the medium supplemented with 4 mg/L of IBA. Ninety six percent of the regenerated plants survived when transferred to soil under glasshouse conditions.