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Threonine synthase (TS) catalyzes the hydrolysis of O-phospho-l-homoserine (OPHS) to produce l-threonine (l-Thr) and inorganic phosphate. Here, we report a simplified purification protocol for the OPHS substrate and a continuous, coupled–coupled, spectrophotometric TS assay. The sequential actions of threonine deaminase (TD) and hydroxyisocaproate dehydrogenase (HO-HxoDH) convert the l-Thr product...
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