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Current methods for measuring fructan levels in plant tissues are time-consuming and costly. They often involve multiple or sequential extractions, enzymatic or acid hydrolysis of fructan polymers, and multiple HPLC runs to quantify fructan-derived hexoses. Here we describe a new method that requires a single extraction step, followed by selective precipitation of fructans by acetone, acid hydrolysis...
In the current work, direct competitive enzyme-linked immunosorbent assay (ELISA) was developed for derivatized vitamin B 12 by generating chicken egg yolk immunoglobulins (IgY) against derivatized vitamin B 12 and purified using affinity chromatography. Checkerboard assay was performed with vitamin B 12 antibody and vitamin B 12 –alkaline phosphatase conjugate followed...
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