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Here we report the first application of amino acid-type selective (AATS) isotope labeling of a recombinant protein secreted by Brevibacillus choshinensis for a nuclear magnetic resonance (NMR) study. To prepare the 15 N-AATS-labeled protein, the transformed B. choshinensis was cultured in 15 N-labeled amino acid-containing C.H.L. medium, which is commonly used in the Escherichia coli...
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