Thin film composite reverse osmosis (TFC RO) membranes (1m breadth×90m length) were prepared by coating polyamide active layer over a porous polysulfone membrane using indigenous membrane casting and coating devices. The TFC membranes exhibited 56±9l/m 2 h water flux and 96.7±0.6% salt rejection when tested with 2000ppm NaCl feed at 250psi pressure. The membrane structural information obtained using SEM, AFM, SANS, PALS, QENS, permeation experiments with organic markers, ATR-IR, and zeta-potential measurements was correlated with the variance in membrane flux and selectivity.