Two new trace saponins were isolated from Oxytropis bicolor by column chromatography and preparative TLC on silica gel. The structures were elucidated as 16-O-[β-d-glucopyranosyl(1 → 3)-β-d-glucopyranosyl]-(20S, 24S)-3β,16β,20,24,25-pentahydroxy-9,19-cyclolanostane and 16-O-[β-d-glucopyranosyl(1 → 3)-β-d-glucopyranosyl-20-O-α-l-arabinopyranosyl-(20S, 24S)-3β,16β,20,24,25-pentahydroxy-9,19-cyclolanostane on the basis of chemical evidence and spectral analysis.