Ten popular species of both edible and medicinal Korean mushrooms were analysed for their free amino acids and disaccharides. The average total free amino acid concentration was 120.79mgg −1 in edible mushrooms and 61.47mgg −1 in medicinal mushrooms, respectively. The average total of free amino acids for all mushrooms, edible mushrooms and medicinal mushrooms was 91.13mgg −1 . Agaricus blazei (227.00mgg −1 ) showed the highest concentration of total free amino acids; on the other hand, Inonotus obliquus (2.00mgg −1 ) showed the lowest concentration among the 10 species of mushrooms. The average total carbohydrates concentration was 46.67mgg −1 in the 10 species of mushrooms, where the edible mushrooms contained 66.68mgg −1 and the medicinal mushrooms contained 26.65mgg −1 . The carbohydrates constituents of the 10 mushroom species were mainly mannose (36.23%), glucose (34.70%), and xylose (16.83%).