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We describe the antagonistic properties due to the replacement of Pro 3 by phenylalanine in porcine motilin. The analogue, [Phe 3 ,Leu 13 ]porcine motilin (OHM-11526), displaces iodinated [Nle 13 ]porcine motilin bound to a homogenate of rabbit antral smooth muscle tissue. The dissociation constant (pK d ) was 9.26 ± 0.04, versus 9.11 ± 0.01 for...
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