The objective of this study was to measure gender-related changes of otoacoustic emissions level in subjects with normal hearing in tonal audiometry.Click evoked otoacoustic emissions (CEOAEs) and distortion-product otoacoustic emissions (DPOAEs) were recorded in 146 (n = 292 ears) subjects. Otoacoustic emissions were recorded in response to nonlinear clicks and two different primary tones (L1 = 65, L2 = 55 dB SPL; f2/f1 = 1.22) from 1 to 5 kHz. Subjects were divided into two groups: female (29.4 ± 12.2 years) and male (27.5 ± 10.1 years). All subjects had normal hearing and middle-ear function based upon standard audiometric criteria.The principal findings were that CEOAEs and DPOAEs levels were smaller in the male group compared to the female group, especially at the higher frequencies of DPOAEs.The influence of gender on the level of otoacoustic emissions was found.