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The molecular, biochemical, and cellular characterization of EhGEF1 protein is described. Complete cDNA sequence of 1890bp revealed an open reading frame that encodes a protein of 69kDa. EhGEF1 is constituted of Dbl homology domain, pleckstrin homology domain, and several putative regulation sites. Studies of guanine nucleotide exchange activity of EhGEF1 on several GTPases from Entamoeba histolytica...
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