Higher urinary sulfate is associated with a favorable cardiovascular profile in renal transplant recipients. Sulfate can be derived from the intake of sulfur-containing amino acids, but is also an end product of, and thus may reflect, hydrogen sulfide metabolism. Since cardiovascular problems are likely to originate pre-transplantation, we hypothesized that the same association could be found for sulfate levels in dialysate of patients with end stage renal disease. Data were taken from a cohort of 29 patients who underwent 240min of standard hemodialysis. Patient characteristics, including protein catabolic rate (PCR) and data from several time points on dialysis and hemodynamic parameters were available. Sulfate levels in dialysate from 6 time points were measured by Ion Chromatography and total excretion was determined by computing the area under the curve (1.10±0.475mmol/L). No associations were found between levels of sulfate in dialysate and hemodynamic parameters (systolic and diastolic blood pressure, mean arterial pressure and heart frequency), although we did observe positive trends. Above average sulfate excretion was found to associate with a higher PCR (+0.212 ±0.0589g/kg/day, p=0.0012). Also, total sulfate excretion correlated with total ultrafiltration volume (R=0.492, p=0.007). We thus conclude that dialysate sulfate levels relate to a favorable nutritional intake, rather than to hemodynamics, in hemodialysis patients.