In this article, we report the formation of two kinds of laser induced periodic surface structures (LIPSSs) with direction perpendicular to laser polarization on the Fe films irradiated by 800nm femtosecond laser pulses. High-spatial frequency LIPSSs (HSFLs) with periods of 150–230nm are observed with small laser pulse number less than 100. Low-spatial frequency LIPSSs (LSFLs) with periods of 500–640nm appear abruptly when increasing the pulse number to a specific pulse number varied with laser fluence, and the periods of LSFLs have a tendency to decrease when the pulse number exceeds some specific values varied for different laser fluences. The formation of high- and low-spatial frequency periodic structures may be related to the surface plasmon polaritons excited by laser on surface iron oxides film or iron film.