To validate the BLDType Multiplex Typing Kit (ThermoFisher) for genotyping hematopoietic stem cell (HSC) registry donors for ABO, RHD and CCR5 using Fragment Analysis (FA).Ninety-one archived genomic DNA samples (gDNAs) that had prior serology and/or ABO, RHD or CCR5 genotyping were typed using BLDType which employs fragment analysis using ABI 3130XL and Genemapper software 5 to distinguish A1, A2, B, O1, O2 and O3 alleles, detects deletion of RHD and CCR5Δ32. Fifty were typed for ABO and CCR5 alleles by restriction fragment length polymorphism (RFLP) and sequence-specific primer (SSP) PCR assays respectively. Twelve were genotyped for CCR5 by SSP and standard tube or automated red blood cell agglutination methods were used for ABO and RhD typing. Concordance rates were calculated for each sample subset as follows: 79 specimens with ABO and RhD phenotyping and 62 specimens with ABO by RFLP.Concordance between RhD serology and FA was 100% (79/79). Concordance between ABO serology and FA was 96% (76/79). The concordance between ABO RFLP and FA was 97% (60/62). The concordance between CCR5 genotyping by FA and SSP methods was 100% (91/91). The discrepancies encountered in this study were limited to ABO and were due to lack of serologic subtyping to distinguish A1 from A2 (N=3) and a limitation of the RFLP which does not detect O3 alleles (N=2) or cannot resolve unusual banding patterns. The BLDType technique is user friendly and easy to implement. FA of 96 gDNAs can be completed in 8h, and if automated and performed on a 3730XL, more than 1000 gDNAs can be tested per day.The validation data presented here demonstrates that BLDType is a highly sensitive and specific typing tool. Incorporating BLDType along with HLA typing for registry donors would expedite the selection processes for both HLA-matched and ABO-compatible donors simultaneously.