Exopolysaccharide (EPS) producing Weissella cibaria CMGDEX3 was isolated from cabbage on sucrose containing De Man, Rogosa and Sharpe (MRS) agar. Dextransucrase activity and dextran yield was found to be 7.1DSUml −1 and 2.4gdl −1 , respectively. The structural characterization of purified EPS determined by FTIR, 1 H and 13 C NMR spectroscopy demonstrated that W. cibaria CMGDEX3 synthesized a linear dextran that predominately had α (1→6) glycosidic linkages with only a few (3.4%) α (1→3) linked branches. Molecular mass determination showed that it was a high molecular weight dextran of an average >2,000,000Da. According to our knowledge this is the first report on isolation of dextran synthesizing Weissella genus from Pakistan.