Dissolved organic carbon (DOC) transported from the soil surface is stabilized in deeper soil profiles by physico-chemical sorption processes. However, it is unclear how different forms of organic carbon (OC) compounds common in soil organic matter interact with soil minerals in the surface (A) and subsurface (B) horizons. We added four compounds (glucose, starch, cinnamic acid and stearic acid) to the silt- and clay-sized fraction (fine fraction) of A and B horizons of eight soils from varying climates (3 temperate, 3 tropical, 1 arctic and 1 sub-arctic). Equilibrium batch experiments were conducted using 0 to 100mgCL −1 of 14 C-labeled compounds for 8h. Sorption parameters (maximum sorption capacity, Q max and binding coefficient, k) calculated by fitting sorption data to the Langmuir equation showed that Q max of A and B horizons was very similar for all compounds. Both Q max and k values were related to sorbate properties, with Q max being lowest for glucose (20–500mgkg −1 ), highest for stearic acid (20,000–200,000mgkg −1 ), and intermediate for both cinnamic acid (200–4000mgkg −1 ) and starch (400–6000mgkg −1 ). Simple linear regression analysis revealed that physico-chemical properties of the sorbents influenced the Q max of cinnamic acid and stearic acid, but not glucose and starch. The sorbent properties did not show predictive ability for binding coefficient k. By using the fine fraction as sorbent, we found that the mineral fractions of A horizons are equally reactive as the B horizons irrespective of soil organic carbon content.