A series of new two-photon pumped (TPP) up-conversion lasing dyes, named PSPI, DEASPI, HMASPI and HEASPI, have been synthesized. These dyes are stilbene-type chromophores end-capped with the same acceptor group but with varied donor groups. Pumped by a 1064nm, ~40ps mode-locked Nd/YAG laser pulses, all chromophores showed TPP up-conversion laser emission with the efficiencies as high as 7.1-10.7%, showing their great potentiality in the application of two-photon areas. Semiempirical calculations showed those chromophores which possess large two-photon absorption (TPA) cross-sections. The relation between TPA and TPP as well as some mechanism were discussed.