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A model for the rapid assessment of iron availability was developed that combines in vitro digestion with iron uptake by Caco-2 cell monolayers. In this method, samples (beef, ascorbic acid, or citric acid) were adjusted to pH 2, labeled with 59 Fe, and subjected to pepsin digestion (pH 2, 37°C) for 1 h to simulate gastric digestion. Next, a dialysis bag (12,000-14,000 molecular weight...
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