This paper deals with an experimental analysis of freezing processes using a horizontal plate freezer with CO 2 as refrigerant. The CO 2 is supplied from an experimental CO 2 /NH 3 cascade refrigeration system facility with a 9 kW of refrigeration capacity at −50 °C. The experimental facility, the data acquisition system and the experimental methodology are described. The experimental results include the temperature evolution in the product to be frozen, the CO 2 evaporating temperature and freezing times. Results show a clear link between the freezing time and CO 2 average evaporating temperature. The observed trend showed that a temperature reduction of 1 °C causes a reduction of approximately 2 min in the freezing time. The experimental results also include the refrigeration capacity of the facility, the electric power of the compressors and the cascade system COP. In addition, a numerical model of the freezing process using a commercial software was developed and validated. The results obtained would confirm that the use of CO 2 as refrigerant in plate freezers represents a viable alternative to the refrigerants currently in use.