This study was conducted to investigate Taiwan University students' perceptions (including experience, confidence and preference) of online academic help seeking (OAHS) and students' level of web-based learning self-efficacy (WLSE). Two instruments, OAHS questionnaire, consisting of information searching, formal and informal query scales, and WLSE questionnaire, including general and functional scales, were then validated through collecting the responses from 300 university students. Results indicate reciprocal relations between experience, confidence and preference in students' online academic help seeking behaviors. Students' academic help seeking behaviors were related to their general self-efficacy in a web-based course setting. Students' functional WLSE was related to their perceptions of information searching for OAHS. Findings of this study also imply that students' experience of seeking help from informal online channels is prominent when they participate in a web-based course.