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Actinidia deliciosa var. deliciosa a dioecious plant species has become very popular for commercial cultivation in the low and mid-Himalayan regions of India. But dioecy represents an important constraint in kiwifruit breeding programmes and also requires identification of male and female genotypes before planting an orchard. The PCR-based RAPD technique can be used to detail the genetic control of...
A combined method of sequence characterized amplified regions (SCAR) primers and Touchdown-PCR was used for the development of a female DNA marker in Pistacia vera L. The random decamer primer OPO-08 amplified a 905-bp fragment in all female trees, but also in several males. SCAR primers designed on the basis of the RAPD female molecular marker amplified a 905-bp female and a 909-bp male fragment...
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