Eight species of Chrysolina have been chromosomally studied in male individuals from Central Europe, South France and the Urals, in Russia. Ch. rufa had 2n = 23, Ch. purpurascens crassimargo 2n = 24 and a meioformula of 11 + Xy p , Ch. globosa and Ch. bigorrensis 12 + Xy p , Ch. umbratilis 2n = 30 and 14 + XY p , a new non-chiasmate sex-chromosome system with both male sex-chromosomes of large size, Ch. cf. subcostata 12 + XY p with large sex-chromosomes also, Ch. interstincta 2n = 40 and 19 + Xy p , and Ch. obscurella 2n = 47. The taxonomic implications of these findings are discussed in relation with the current subgeneric classification of Chrysolina and principally that of the subgenera Colaphoptera, Sphaerochrysolina, Pleurosticha, Chalcoidea and Threnosoma.