A new copper carbon paste electrode (CPE) based on incorporation bis(2, 4-dihydroxybenzyliden)-1,6-diaminohexane (DHBDAH) in graphite powder matrix has been described. The influence of variables including an amount of graphite, sodium tetraphenylborate (NaTPB), DHBDAH and nujol on the Cu 2+ carbon paste electrode response was studied and optimized. The optimum carbon paste composition was set as follows, graphite powder: NaTPB: Nujol: DHBDAH with amount of 150:2.3:30:4mg, respectively. At the optimum conditions, the potential response is linear over the concentration range of 5.0×10 −8 to 1.0×10 −1 molL −1 with a Nernstian slope of 29.5±1.1mV per decade of Cu 2+ ion concentration. The good performance of electrode such as low detection limit of (LOD) (4×10 −8 molL −1 ), wide applicable pH range (2.5–5.5), fast response time (˂10s) and adequate shelf life (69days) indicate the utility of the proposed electrode for evaluation of Cu 2+ ion content in various analysis. Due to moderate potentiometric selectivity coefficients of proposed electrode obtained by fixed interference method (FIM) and separate solution method (SSM), the proposed electrode successfully can be applied for the determination of Cu 2+ ions content in some real samples.