Biohydrogen production from probiotic wastewater using mixed anaerobic consortia is reported in this paper. Batch tests are carried out in a 5.0L batch reactor under constant mesophillic temperature (37°C). The maximum hydrogen yield 1.8mol-hydrogen/mol-carbohydrate is obtained at an optimum pH of 5.5 and substrate concentration 5g/L. The maximum hydrogen production rate is 168ml/h. The hydrogen content in the biogas is more than 65% and no significant methane is observed throughout the study. In addition to hydrogen, acetate, propionate, butyrate and ethanol are found to be the main by-products in the metabolism of hydrogen fermentation.