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HlyT from Vibrio cholerae is a positive regulator of Na + /H + antiporter, important for the survival of the organism in an aquatic environment and within the human host. Here we report cloning, over-expression and purification of HlyT. Analytical gel filtration and glutaraldehyde cross-linking indicate existence of tetrameric and dimeric forms of HlyT in solution. We propose an unfolding...
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