In the present study, pyrolysis of Mesua ferrea seed cover (MFSC) and Pongamia glabra seed cover (PGSC) was performed to investigate the characteristics of bio-oil and its sub fractions. In a fixed bed reactor, the effect of temperature (range of 350–650°C) on product yield and quality of solid product were monitored. The maximum bio-oil yield of 28.5wt.% and 29.6wt.% for PGSC and MFSC respectively was obtained at 550°C at heating rate of 40°C/min. The chemical composition of bio-oil and its sub fractions were investigated using FTIR and 1 H NMR. GC–MS was performed for both PGSC and MFSC bio-oils and their corresponding n-hexane fractions. The results showed that bio-oil from the feedstocks and its sub-fractions might be a potential source of renewable fuel and value added chemicals.